An ELISA parvo test is one of the most common methods of detecting the presence of parvovirus in puppies. ELISA stands for Enzyme Linked ImmunoSorbant Assay and is performed using the same technology used in home pregnancy tests. An ELISA parvo test can be done in your vet's office in about 15 minutes. It is a highly sensitive test, which detects traces of the virus in the stool. When there is a positive test result, it indicates that your puppy has active parvo infection.
In recent years, vaccinations have become an important part of routine care, so vaccination of puppies has decreased the prevalence of parvovirus infection. However, many puppies have recently received vaccinations, and if they have not yet developed antibodies, they can still become infected. The ELISA parvo test is useful in identifying puppies who have recently been vaccinated. While a negative ELISA parvo test results in the majority of cases, it is always best to treat your puppy if the results are positive.
The ELISA parvo test is sensitive, but it can also produce false results. A dog will not shed parvovirus until four to five days after exposure. If a dog was vaccinated recently, antigen testing will produce a false-negative result. Moreover, the vaccine contains modified-live virus and the ELISA parvo test may result in a false-positive result.
An ELISA parvo test can be performed in the veterinarian's office within 15 minutes. It is highly sensitive to parvovirus, a virus which is spread via feces. A dog's feces contain the antibodies for the parvovirus, which is detected with an ELISA test. A positive result is accompanied by a color-changing reaction. Most ELISA parvo tests can be completed in less than fifteen minutes.
The SNAP parvo test uses enzyme-linked immunosorbent assay technology. The FASTest parvo strip utilizes a sandwich system of two antibodies - one membrane-fixed and the other bound to gold particles. Witness parvo card uses immunomigration technology. Color changes on the nitrocellulose strip indicate the presence of antigen-antibody complexes. In the study, the SNAP ELISA test detected 78% of parvovirus strains.
If you have any doubts about your dog's health, contact your veterinarian immediately. The dog's parvo test may be a false positive if your dog has recently had a parvo vaccination. To avoid a false-positive result, you should inform your veterinarian that your dog has received the vaccine within the last five or twelve days. The test is accurate when your veterinarian confirms the presence of parvovirus.
The CPV ELISA detection kit, developed by CSL Ltd. in Australia, uses a novel concept in enzyme immunoassay. The conjugate antibody is lyophilised together with the solid-phase capture antibody. Its sensitivity and specificity were comparable to that of haemagglutination assay, which requires electron microscopy and virus isolation. The CPV ELISA proved to be superior in this regard.
There maybe some residual substances on the ELISA plate after the detetion. In order to reduce the errors caused by the residues, an ELISA Washer is needed. Elisa washer is a medical device specially designed to clean the microplate and generally used in conjunction with the microplate reader. It has been widely used in the cleaning of ELISA plates in hospitals, blood stations, health and epidemic prevention stations, reagent factories and research laboratories.